HYBRID EVENT: You can participate in person at Rome, Italy or Virtually from your home or work.

2nd Edition of Virology World Conference

21-22, 2023

June 21 -22, 2023 | Rome, Italy


Speaker at Virology World Conference 2023 - Zucatelli
Instituto Butantan, Brazil
Title : Antiviral action of aqueous extracts of propolis from scaptotrigona aff. postica against zica, chikungunya, and mayaro virus


The limited availability of antivirals for new highly pathogenic strains of virus has become a serious public health problem that kills thousands of people annually. For this reason, the search for new products against these agents has become an urgent necessity. Many studies have been carried out with this aim. Among the multiple sources of research for new antibiotics and antivirals, bioprospecting from insect exudates or their products has become an increasingly frequent option. Insects appeared on the planet about 350 million years ago and have been one of the beings with greater adaptability and resistance to the most varied biomes. Insects have been found in all known ecosystems. Their survival for so long, in such different environments, is an indication that they have a very efficient protection system against environmental infections, despite not having a developed immune system like mammals. Historically, since the ancient civilizations of Egypt and Rome, the products obtained from the bee, such as honey and propolis, have been of great pharmacological importance, being used as antimicrobial, antiinflammatory, antitumor, healing several other functions. Investigations on the chemical composition and biological activity of propolis have been carried out, mainly in the species Apis mellifera, and this product has shown activity against some important viruses, such as influenza, poliovirus, hepatitis, HIV, and others. However, for the Meliponini species, known as stingless bees, there are few studies, either on their chemical composition or on their biological activities. The importance of studying these bees is because they come from regions with native forests, and therefore with many species of plants not yet studied, in addition to which they are regions still free of pesticides, which guarantees a greater fidelity of the obtained data. Previous studies by our group with crude hydroalcoholic extract of propolis demonstrated an intense antiviral activity against Herpes, influenza, and rubella viruses. All studies carried out with propolis are done with alcoholic extracts. In this work, we chose to use aqueous extracts, which eliminates the presence of other compounds besides those originally present in propolis, in addition to extracting substances different from those obtained in alcoholic extracts, which makes this work unprecedented. Therefore, this study aimed to identify, isolate and characterize compounds with antiviral effects from aqueous propolis extracts from Scaptotrigona aff postica,  in emerging viruses such as zicavirus, chikungunya, and Mayaro. The evaluation of the antiviral activity of the crude and purified material was performed by reducing infectious foci in cultures of infected cells treated with propolis extracts in infected cultures and treated or not treated with propolis. The chemical characterization of the elements present in the extracts was performed by liquid chromatography. The results obtained indicate a high reduction of Zicavirus, chikungunya, and Mayaro virus replication (average 100x) when compared to infected-only cultures. Likewise, it was observed that the antiviral response was dose-dependent, being more intense when propolis was added 2 hours after the viral infection.

Audience take away: 

  • Explain how the audience will be able to use what they learn.
  • How will this help the audience in their job?
  • Is this research that other faculty could use to expand their research or teaching?
  • Does this provide a practical solution to a problem that could simplify or make a designer’s job more efficient?
  • Will it improve the accuracy of a design, or provide new information to assist in a design problem?
  • List all other benefits.


Dr. Edward studied Chemistry at the Sofia University, Bulgaria and graduated as MS in 1999. She then joined the research group of Prof. James at the Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences (IGIC-BAS). She received her PhD degree in 2004 at the same institution. After one year supervised by Dr Williams at the Catalysis and Spectrochemistry Laboratory, France she obtained the position of an Associate Professor at the IGIC. S he has published more than 70 research articles in SCI(E) journals.)

Dr. Zucatelli studied Pharmacy and Biochemistry at Londrina University (Brazil), Master and Doctorate in Microbiology at University of São Paulo. He held three postdoctoral fellowships in biotechnology.  Scientific researcher at Instituto Butantan since 1984. Worked with production and research of anti-tetanus, anti-botulinum and gangrene vaccines. Has experience in ​​Microbiology, with emphasis on Virology, biotechnology developed and optimized the production of an anti-rabies vaccine in vero cells in microcarriers. Work with  culture of animal and insect cells, apoptosis, cellular metabolism. Development of viral vaccines, optimization of processes and bioreactor for the production of vaccine viruses in microcarriers and development of culture media. Isolation, purification and cloning of bioactive substances from hemolymph of arthropods, ophidians, molluscs, propolis and plants, in particular antiviral, antimicrobial, antitumor and anti-inflammatory drugs. He is currently Director of the Parasitology Laboratory at the Butantan Institute.  He has published more than 90 research articles in SCI(E) journals and more than 250 congress.